水稻类病变突变体g303的鉴定和基因定位

doi:10.3969/j.issn.1001-7216.2014.05.003

中国水稻科学 ›› 2014, Vol. 28 ›› Issue (5): 465-472.DOI: 10.3969/j.issn.1001-7216.2014.05.003

水稻类病变突变体g303的鉴定和基因定位

刘林^1,# ,张迎信^1,# ,李枝^1,2 ,刘群恩¹,余宁¹ ,孙滨¹ ,杨正福¹ ,周全¹ ,程式华¹,曹立勇^1,*

¹中国水稻研究所/国家水稻改良中心/浙江省超级稻研究重点实验室，杭州 310006； ²杭州师范大学生命与环境科学学院，杭州 310036；

收稿日期:2014-01-16 修回日期:2014-02-17 出版日期:2014-09-10 发布日期:2014-09-10
通讯作者: 曹立勇1,*
基金资助:
国家转基因重大专项（2011ZX08001002）；国家自然科学基金资助项目（31071398, 31101203）。

Characterization and Gene Mapping of a Lesion Mimic Mutant g303 in Rice

LIU Lin ^{1, #} , ZHANG Yingxin ^{1, #} , LI Zhi ^1,2 , LIU Qunen ¹ , YU Ning ¹, SUN Bin ¹, YANG Zhengfu ¹, ZHOU Quan ¹, CHENG Shihua ¹ , CAO Liyong ^1,*

¹Zhejiang Key Laboratory of Super Rice/ National Center for Rice Improvement/China National Rice Research Institute, Hangzhou 310006, China; ²College of Life and Environmental Sciences, Hangzhou Normal University, Hangzhou 310036, China;

Received:2014-01-16 Revised:2014-02-17 Online:2014-09-10 Published:2014-09-10
Contact: CAO Liyong1,*

摘要/Abstract

摘要： 通过γ射线辐射诱变籼稻9311和中恢8015分别获得了2个类病变突变体g303和g342。g303播种4~5周叶片开始出现黄褐色斑点。随着植株的生长发育，至成熟期病斑几乎布满整个植株。与野生型相比，突变体g303株高、结实率、千粒重显著下降，叶绿素含量、光合速率显著降低。遗传分析表明，g303类病变性状由一对隐性基因控制。利用g303/日本晴F2群体进行定位，将突变基因定位于第12染色体标记InD10和InD12之间，遗传距离分别为0.19 cM和0.76 cM。测序分析发现，该基因与SL基因等位，其中g303和g342分别在编码区第572位和第1206位发生单碱基缺失，导致翻译提前终止。实时RTPCR结果显示抗病相关基因在突变体g303中表达量显著上升，说明该基因突变很可能激活了防卫反应。

关键词: 水稻, 类病变突变体, 基因定位, 防卫反应

Abstract: The lesion mimic mutant g303 and g342 were isolated by treating the seeds of indica varieties 9311 and R8015 with γray radiation, respectively. The yellow spots initially appear on g303 leaves at 4leaf stage. With the growth and development of the plant, the lesions almost occupied the whole plant till the maturity stage. Compared with the wild type, the plant height, seed setting rate and 1000grain weight of g303 significantly dropped, the chlorophyll and photosynthetic rate obviously decreased. Genetic analysis showed that the mutant phenotype was controlled by a single recessive gene. Using F2 mapping population of g303/ Nipponbare, the g303 mutant gene was mapped between marker InD10 and InD12 on rice chromosome 12, with genetic distances of 0.19 cM and 0.76 cM, respectively. Sequence analysis revealed that the mutated gene was allelic to SL, g303 and g342 mutated gene had a single nucleotide deletion (T572 and G1206, respectively), leading to a premature termination codon. Realtime PCR analysis showed that the expression level of defenserelated genes upregulated significantly, these results demonstrated that the mutation may activate the defense response.

Key words: rice, lesion mimic mutant, gene mapping, defense response

中图分类号:

Q343.5
Q754

刘林1,# ,张迎信1,# ,李枝1,2 ,刘群恩1 ,余宁1 ,孙滨1 ,杨正福1 ,周全1 ,程式华1 ,曹立勇1,*. 水稻类病变突变体g303的鉴定和基因定位[J]. 中国水稻科学, 2014, 28(5): 465-472.

LIU Lin1, #, ZHANG Yingxin1, #, LI Zhi1,2, LIU Qunen1, YU Ning1, SUN Bin1, YANG Zhengfu1, ZHOU Quan1, CHENG Shihua1, CAO Liyong1,*. Characterization and Gene Mapping of a Lesion Mimic Mutant g303 in Rice[J]. Chinese Journal of Rice Science, 2014, 28(5): 465-472.

参考文献

\[1\]Hoisington D A, Neuffer M G, Walbot V. Disease lesion mimics in maize: Ⅰ. Effect of genetic background, temperature, developmental age, and wounding on necrotic spot formation with Les1. Dev Biol, 1982, 93(2): 381388.

\[2\]Dietrich R A, Richberg M H, Schmidt R,et al. A novel zinc finger protein is encoded by the Arabidopsis LSD1 gene and functions as a negative regulator of plant cell death. Cell, 1997, 88: 685694.

\[3\]Gray J, Close P S, Briggs S P, Johal G S. A novel suppressor of cell death in plants encoded by the lls1 gene of maize. Cell, 1997, 89: 253l.

\[4\]Buschges R, Hollricher K, Panstruga R,et al. The barley mlo gene: A novel control element of plant pathogen resistance. Cell, 1997, 88: 695705.

\[5\]Yamanouchi U, Yano M, Lin H X,et al. A rice spotted leaf gene, Spl7, encodes a heat stress transcription factor protein. Proc Natl Acad Sci, 2002, 99: 75307535.

\[6\]Dietrich R A, Delaney T P, Uknes S J, et al. Arabidopsis mutants simulating disease resistance response. Cell, 1994, 77(4): 565577.

\[7\]Takahashi A, Kawasaki T, Henmi K, et al. Lesion mimic mutants of rice with alterations in early signaling events of defense. Plant J, 1999, 17(5): 535545.

\[8\]Mizobuchi R, Hirabayashi H, Kaji R, et al.Differential expression of disease resistance in rice lesionmimic mutants. Plant Cell Rep, 2002, 21: 390396.

\[9\]Zeng L R, Qu S H, Bordeos A,et al. Spotted leaf11, a negative regulator of plant cell death and defense, encodes a ubox/armadillo repeat protein endowed with E3 ubiquitin ligase activity. Plant Cell, 2004, 16: 27952808.

\[10\]Mori M, Tomita C, Sugimoto K, et al. Isolation and molecular characterization of a Spotted leaf 18 mutant by modified activationtagging in rice. Plant Mol Biol, 2007, 63: 847860.

\[11\]Fujiwara T, Maisonneuve S, Isshiki M,et al. Sekiguchi lesion gene encodes a cytochrome P450 monooxygenase that catalyzes conversion of tryptamine to serotonin in rice. J Biol Chem, 2010, 285: 1130811313.

\[12\]Sun C, Liu L, Tang J,et al. RLIN1,encoding a putative coproporphyrinogen III oxidase, is involved in lesion initiation in rice. J Genet Genom, 2008, 38: 2937.

\[13\]Chen X, Hao L, Pan J, et al.SPL5, a cell death and defenserelated gene, encodes a putative splicing factor 3b subunit 3 (SF3b3) in rice. Molecul Breeding, 2012, 30(2): 939949.

\[14\]Undan J R, Tamiru M, Abe A, et al. Mutation inOsLMS, a gene encoding a protein with two doublestranded RNA binding motifs, causes lesion mimic phenotype and early senescence in rice (Oryza sativa L.). Genes Genet Syst, 2012, 87(3): 169179.

\[15\]Wellburn A R. The spectral determination of chlorophyll a and b, as well as total carotenoids, using various solvents with spectrophotometers of different resolution. J Plant Physiol, 1994, 144: 307313.

\[16\]Rogers S O, Bendich A J. Extraction of DNA from milligram amounts of fresh, herbarium and mummified plant tissues. Plant Mol Biol, 1985, 5: 6976.

\[17\]Lorrain S, Vailleau F,Balague Roby D. Lesion mimic mutants: Keys for deciphering cell death and defense pathways in plants? Trends Plant Sci, 2003, 8: 262271.

\[18\]李秀兰, 王平荣, 曲志才, 等. 水稻类病变突变体C23的遗传分析与基因的精细定位. 中国农业科学, 2010, 43(18): 36913697.

\[19\]杨绍华, 刘华清, 王锋. 水稻斑点叶突变体W1764的遗传分析及初步定位. 福建农业学报, 2011, 26(4): 519522.

\[20\]吴超, 付亚萍, 胡国成, 等. 一个水稻类病变黄叶突变体的鉴定和精细定位. 中国水稻科学, 2011, 25(3): 256260.

\[21\]陈萍萍, 叶胜海, 赵宁春, 等. 浙粳22类病斑突变体spl(t)特征及其基因定位. 核农学报, 2010, 24(1): 00010006.

\[22\]Penning B W, Johal G S, McMullen M D. A major suppressor of cell death,slm1, modifies the expression of the maize ( Zea mays L.) lesion mimic mutation les23. Genome, 2004, 47: 961969.

\[23\]Yin Z C, Chen J, Zeng L R, et al. Characterizing rice lesion mimic mutants and identifying a mutant with broadspectrum resistance to rice blast and bacterial blight.Mol Plant Microbe In, 2000, 13(8): 869876.

\[24\]Ishihara A, Hashimoto Y, Tanaka C, et al. The tryptophan pathway is involved in the defense responses of rice against pathogenic infection via serotonin production. Plant J, 2008, 54: 481495.

\[25\]Thomma B P, Penninckx I A, Broekaert W F, et al. The complexity of disease signaling in Arabidopsis. Cur Opi Immun, 2001, 13: 6368.

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水稻类病变突变体g303的鉴定和基因定位

Characterization and Gene Mapping of a Lesion Mimic Mutant g303 in Rice

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